90-05-1 | Guaiacol

Chemical type

• Phenolic compound (2-methoxyphenol)
• Electron-donating substrate for peroxidases

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• Phenolic oxygenated aromatic compound (lignin-derived monomer)

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Key properties

• Used in peroxidase activity assay at 60 μmoles concentration
• Forms colored product (tetraguaiacol) upon oxidation, measured at 470 nm
• Km value with PLPOD: 0.067 mM (high affinity)

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• Contains two oxygen-containing functional groups: hydroxyl (–OH) and methoxy (–OCH₃)
• Representative compound derived from lignin in biomass
• High oxygen content compared with petroleum hydrocarbons
• Often used as a model compound for bio-oil upgrading studies

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• Chromogenic substrate for peroxidase activity measurement
• Electron donor in enzymatic assays for plant physiology and stress studies
• Indicator in colorimetric detection of H₂O₂

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• Model molecule for studying biomass upgrading and catalytic hydrodeoxygenation
• Intermediate in conversion of biomass to fuels and chemicals

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Classification by use

• Chemicals used as substrates in enzyme assays
• Chemicals used in biochemical research
• Chemicals for oxidative stress analysis

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1. [Cite:1] Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit, Journal of Genetic Engineering and Biotechnology, Volume 23, Issue 4, December 2025, 100584
2. [Cite:2] Hydrodeoxygenation of lignin-derived guaiacol using ruthenium‑titanium-oxide aerogel catalysts, Applied Catalysis O: Open, Volume 212, March 2026, 207088