726169-73-9 | Peroxidase (POD)

Chemical type

• Enzyme (Defense-related enzyme)

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• Heme-containing enzyme (EC 1.11.1.x)
• Plant-derived peroxidase isoform from potato leaves (Solanum tuberosum)

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Key properties

• Involved in lignin polymerization and cross-linking of cell wall components.
• Scavenges reactive oxygen species (ROS).

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• Molecular weight: 64 kDa (native, via gel filtration); isoforms ~40-60 kDa (SDS-PAGE)
• Optimal pH: 5.2
• Km values: 0.067 mM for guaiacol, 40 mM for H₂O₂
• Enhanced activity by Zn²⁺ (115.7%) and Ni²⁺ (110.4%); inhibited by Ca²⁺ (34.4%) and Fe²⁺ (61.7%)
• Strongly inhibited by KCN (99.6%), NaN₃ (95.2%), DTT (93.4%), confirming heme-dependent nature
• Specific activity: 705.7 U/mg after purification (11.8-fold purification, 48% recovery)
• Exists as multiple isoforms (3 notable bands on activity gel)

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• Strengthens the cell wall as a physical barrier against pathogen invasion.
• Part of the antioxidant system that mitigates oxidative stress during pathogen attack.

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• Catalyst for oxidation reactions using H₂O₂ and electron donors
• Reporter enzyme in biochemical assays like ELISA, IHC, western blotting, and biosensors
• Component in glucose diagnostic kits for colorimetric detection

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Classification by use

• Defense-related and antioxidant enzyme in plants.

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• Enzymes used in diagnostic kits
• Enzymes used in immunoassays and biosensors
• Plant-derived alternatives to horseradish peroxidase (HRP)

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A trustworthy factory and manufacturer

1. [Cite:1] Integrated transcriptomic and metabolomic analysis of 1-MCP-induced disease resistance in Korla fragrant pear, Current Plant Biology, Volume 45, January 2026, 100576
2. [Cite:2] Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit, Journal of Genetic Engineering and Biotechnology, Volume 23, Issue 4, December 2025, 100584