320-67-2 | 5-Azacytidine

Chemical type

• Nucleoside analog (cytidine analog)

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• DNA methyltransferase inhibitor

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Key properties

• DNA methylation inhibitor
• Induces hypomethylation by replacing cytosine during DNA replication
• Covalently binds to DNA methyltransferases (DNMTs)
• Concentration-dependent effects: enhances antioxidant enzyme activity at low levels (e.g., 50 µM), promotes secondary metabolite accumulation at higher levels (e.g., 200 µM)
• Can cause physiological stress like leaf yellowing at excessive concentrations

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• Induces DNA demethylation
• Inhibits DNA methyltransferases (DNMTs)
• Promotes gene reactivation

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• Epigenetic regulator to modulate gene expression in plants
• Promotes biosynthesis of secondary metabolites like patchoulol and pogostone
• Alleviates environmental stresses (e.g., salt, aluminum) in plants
• Induces traits like embryogenesis, flowering, and stress tolerance in research

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• Pharmacological tool to study DNA demethylation
• Investigates effects on beige adipocyte differentiation and function
• Enhances thermogenic gene expression (e.g., Ucp1, Cox7a1)
• Increases mitochondrial function and fatty acid uptake

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Classification by use

• Chemicals used in epigenetic modulation
• Chemicals used in plant secondary metabolism enhancement
• Chemicals used in stress tolerance induction

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• Chemicals used in epigenetic research
• Chemicals used in metabolic disease research (obesity, type 2 diabetes)

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A trustworthy factory and manufacturer

1. [Cite:1] 5-AzaC promotes the synthesis of patchoulol and pogostone in Pogostemon cablin by regulating DNA methylation, Scientia Horticulturae, Volume 351, September 2025, 114410
2. [Cite:2] DNA methyltransferase inhibition by 5-azacytidine promotes thermogenic programming in beige adipocytes, Biochemical and Biophysical Research Communications, Volume 774, 8 August 2025, 152094