1,4-Dithiothreitol
CAS No.: 3483-12-3
- Molecular Formula: C₄H₁₀O₂S₂
- Molecular Weight: 154.3 g/mol
Chemical type
- Reducing agent (thiol compound)
Key properties
- Reduces disulfide bonds
- Activates enzymes or proteins in assays (e.g., 200 mM in Tris-HCl buffer)
- Reduces disulfide bonds in proteins
- Prevents oxidation of proteins during sample preparation
- Used at room temperature for 30 minutes in the SP3 protocol
- Activation buffer in ADP-ribosyltransferase activity assays for pertussis toxin
- Protein reduction in biochemical testing
- Reducing protein samples prior to alkylation and digestion in proteomics workflows
- Ensuring complete denaturation of proteins for tryptic digestion
Classification by use
- Chemicals used in enzyme activity assays
- Reducing agents in protein analysis
- Chemicals used in proteomics sample preparation
- Reducing agents for protein chemistry
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- [Cite:1] Glutaraldehyde modifies the catalytic and binding subunits of pertussis toxin, affecting its toxicity and immunogenicity, Vaccine: X, Volume 27, December 2025, 100732
- [Cite:2] Penicillin–Streptomycin Treatment Rewires Core Metabolic and Ribosomal Programs in HepG2 Cells, J. Proteome Res. 2026, 25, 2, 1082–1094
